Three-dimensional traction microscopy accounting for cell-induced matrix degradation
نویسندگان
چکیده
منابع مشابه
Three-Dimensional Traction Force Microscopy: A New Tool for Quantifying Cell-Matrix Interactions
The interactions between biochemical processes and mechanical signaling play important roles during various cellular processes such as wound healing, embryogenesis, metastasis, and cell migration. While traditional traction force measurements have provided quantitative information about cell matrix interactions in two dimensions, recent studies have shown significant differences in the behavior...
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Several biological processes, including cell migration, tissue morphogenesis, and cancer metastasis, are fundamentally physical in nature; each implicitly involves deformations driven by mechanical forces. Traction force microscopy (TFM) was initially developed to quantify the forces exerted by individual isolated cells in two-dimensional (2D) culture. Here, we extend this technique to estimate...
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Talin binds to β-integrin tails to activate integrins, regulating cell migration, invasion and metastasis. There are two talin genes, TLN1 and TLN2, encoding talin1 and talin2, respectively. Talin1 regulates focal adhesion dynamics, cell migration and invasion, whereas the biological function of talin2 is not clear and, indeed, talin2 has been presumed to function redundantly with talin1. Here,...
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Although cell-matrix adhesive interactions are known to regulate stem cell differentiation, the underlying mechanisms, in particular for direct three-dimensional encapsulation within hydrogels, are poorly understood. Here, we demonstrate that in covalently crosslinked hyaluronic acid (HA) hydrogels, the differentiation of human mesenchymal stem cells (hMSCs) is directed by the generation of deg...
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Methods hMSC cell culture. Human mesenchymal stem cells (hMSCs) were obtained from Lonza Corporation (Wakersville, MD). For encapsulation studies, hMSCs were expanded in growth media (α-MEM, 10% FBS, 1% L-Glutamine & penicillin-streptomycin) and encapsulated at passage 3 in MeHA, MeAlg, MeDex (all at 15 x 10 cells mL) or MeMaHA (10 cells mL) hydrogels. For studies evaluating hMSC differentiatio...
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ژورنال
عنوان ژورنال: Computer Methods in Applied Mechanics and Engineering
سال: 2020
ISSN: 0045-7825
DOI: 10.1016/j.cma.2020.112935